en عمومى General پژوهشي Research Background: Brucellosis caused by Brucella spp, is an infectious and zoonotic disease of wild and domestic animals of worldwide distribution. Aim: The present research was conducted to determine the sero-prevalence of brucellosis in humans, Homo sapien (L, 1758) population in Charsadda, Khyber Pakhtunkhwa (KP), Pakistan during August 2014-January 2015. Methods: Blood samples (n=200) were collected randomly during February-December 2014. They were screened for brucellosis by using Serum Plate Agglutination Test (SPAT) and Polymerase Chain Reaction (PCR). Results: Two species, B. abortus and B. melitensis were present separately or in the same sample by SPAT while only separately by PCR. Out of 200 samples of humans 10% and 7.5% were positive by using SPAT and PCR, respectively. The prevalence of the disease in males were, 12% by SPAT while 9% by PCR, in female, 8% by SPAT (males to females’ ratio of 3:2) while 6% by PCR(males to females’ ratio of 4:3). In different age groups of humans, i.e., 1-20, 21-40, 41-60, and >60 were as 10.38, 9.52, 12.00, and 7.14% by SPAT while 7.79, 8.33, 4.00, and 7.14% by PCR, respectively. In professional groups of humans, i.e., farmers, animal keepers, servants, house-wives and others, the prevalence was 11.42, 12.5, 0, 8.75 and 10.71% by SPAT while 8.57, 6.25, 0, 7.5, 7.14 and 3.75% by PCR, respectively. Conclusion: The sero-prevalence of brucellosis in humans is existed in the study area. Recommendation: An effective control programme of the disease is recommended. Brucellosis, infectious, Polymerase Chain Reaction (PCR), Serum Plate Agglutination (SPAT), zoonotic disease 232 240 http://intjmi.com/browse.php?a_code=A-10-1-84&slc_lang=en&sid=1 Farzana Perveen 10031947532846002451 10031947532846002451 Yes Chairperson, Department of Zoology, Shaheed Benazir Bhutto University, Main Campus, Sheringal, Dir Upper, Khyber Pakhtunkhwa, Pakistan Raqeebullah 10031947532846002452 10031947532846002452 No Department of Zoology, Hazara University, Garden Campus, Mansehra-21300, Pakistan Muhammad Shahid 10031947532846002453 10031947532846002453 No Center Microbiology and Biotechnology, Veterinary Research Institute, Peshawar, 25000, Khyber Pakhtunkhwa, Pakistan