en عمومى General پژوهشي Research <pre style="margin-bottom:8px; text-align:justify"> <span style="font-size:10pt"><span courier="" new="" style="font-family:"><b><span style="font-size:14.0pt"><span new="" roman="" style="font-family:" times="">Background:</span></span></b></span></span></pre> <pre style="margin-bottom:8px; text-align:justify"> <span style="font-size:10pt"><span courier="" new="" style="font-family:"><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Root canal sealers are effective in completely filling the root canal by reducing apical and coronal microleakage. The purpose of this study is to compare the bacterial microleakage of two methods of single cone filling and lateral compression with two sealers AH26 and Endoseal MTA.</span></span><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></pre> <pre style="margin-bottom:8px; text-align:justify"> <span style="font-size:10pt"><span courier="" new="" style="font-family:"><b><span style="font-size:14.0pt"><span new="" roman="" style="font-family:" times="">Methods:</span></span></b></span></span></pre> <pre style="margin-bottom:8px; text-align:justify"> <span style="font-size:10pt"><span courier="" new="" style="font-family:"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> 56 single-rooted teeth less than 1 mm from the apical foramen were cleaned and shaped with Sx to F3 files of the Protaper gold system along with washing with 5.25% sodium hypochlorite and sterilized by UV rays. Then they were divided into four groups (n=14) based on the type of sealer (Endoseal MTA or AH26) and canal filling method (side density or single cone). In each group, one positive control sample and one negative control sample were considered.</span></span> <span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Then the canal of the teeth was filled according to their group. The separate compartment model was used to investigate bacterial leakage with enterococcus faecalis bacteria. The samples were placed in an incubator and observed for 90 days. The day of turbidity of the culture medium of each sample was recorded. Finally, the data were analyzed using SPSS version 22 software and through chi-square, Kruskal-Wallis and Kaplan-Meier analyzes by log-rank test.</span></span><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></pre> <pre style="margin-bottom:8px; text-align:justify"> <span style="font-size:10pt"><span courier="" new="" style="font-family:"><b><span style="font-size:14.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b> </span></span></pre> <pre style="margin-bottom:8px; text-align:justify"> <span style="font-size:10pt"><span courier="" new="" style="font-family:"><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">The highest number of teeth that suffered microleakage in the 90-day interval was observed in the Endoseal MTA group using the lateral compression method (91.7%) and the lowest number of microleakage was observed in the AH26 group using the single cone method (66.7%); However, no statistically significant relationship was found between the number of occurrences of microleakage and the studied groups (P=0.468).</span></span></span></span></pre> <pre style="margin-bottom:8px; text-align:justify"> <span style="font-size:10pt"><span courier="" new="" style="font-family:"><b><span style="font-size:14.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b> </span></span></pre> <pre style="margin-bottom:8px; text-align:justify"> <span style="font-size:10pt"><span courier="" new="" style="font-family:"><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Considering the limitations of this study, no difference was observed in the use of AH26 and Endoseal MTA sealers with single cone and lateral compression methods in preventing bacterial microleakage. However, it seems that for Endoseal MTA sealer, using the single cone method will bring better results.</span></span></span></span></pre> Endoseal MTA, AH26, Lateral density, Single cone, Sealer, Bacterial microleakage 1 7 http://intjmi.com/browse.php?a_code=A-10-1-651&slc_lang=en&sid=1 Elham Mahmoudi Email: dr.elhammahmoodi@yahoo.com 10031947532846008202 10031947532846008202 No Assistant Professor of Endodontics ,Department of Endodontics, School of Dentistry, Babol University of Medical Sciences,Mazandaran,Iran Abazar pournajaf abazar_pournajaf@yahoo.com 10031947532846008203 10031947532846008203 No - Department of Microbiology, Faculty of Medicine, Babol University of medical sciences, Babol, Mazandaran,Iran Amir hosein jalounejad jalounejad@gmail.com 10031947532846008204 0009-0004-3112-3705 No Surgeon and Dentist, School of Dentistry, Babol University of Medical Sciences,Mazandaran,Iran Tasnim bagheri tasnim_bagherechenare@gmail.com 10031947532846008205 10031947532846008205 No Assistant Professor of Endodontics, Department of Endodontics, School of Dentistry, Babol University of Medical Sciences,Mazandaran,Iran Seyedali seyedmajidi s.majidi.dvm@gmail.com 10031947532846008206 10031947532846008206 No Dental Materials Research Center, Health Research Institute, Babol University of Medical Sciences, Mazandaran,Iran Mahya Zahra Ghafari mahya.ghafari6@gmail.com 10031947532846008207 0009-0000-3061-9654 Yes Endodontics Resident, Department of Endodontics, School of Dentistry, Babol University of Medical Sciences,Mazandaran,Iran